PLoS ONE (Jan 2017)

Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates.

  • Rebeca M Torrente-Rodríguez,
  • Víctor Ruiz-Valdepeñas Montiel,
  • Susana Campuzano,
  • María Pedrero,
  • Meryem Farchado,
  • Eva Vargas,
  • F Javier Manuel de Villena,
  • María Garranzo-Asensio,
  • Rodrigo Barderas,
  • José M Pingarrón

DOI
https://doi.org/10.1371/journal.pone.0175056
Journal volume & issue
Vol. 12, no. 4
p. e0175056

Abstract

Read online

The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.