Improved Swiss-rolling method for histological analyses of colon tissue

Abstract

Since the introduction of the Swiss-rolling technique by Reilly and Kirsner in 1965, various methodological approaches have been developed for histological analyses of intestinal tissues. Here, we describe an improved protocol for the processing of freshly harvested murine colons that can be extended to other intestinal tissues. With simple tools, this technique allows to tightly wrap the organ throughout the whole length and to keep it in place before fixation, avoiding excessive stiffness of the tissue. Unlike the original method which relies on frozen samples, processing of the biological samples right after resection preserves epitopes integrity for subsequent immunohistochemical analyses. Ultimately, this method provides a reproducible workflow to capture the entire colon length in a unique histological section in order to assess several features such as intestinal inflammation and tumorigenesis.• Easily include freshly isolated tissues• Shorten preparation time using a few affordable tools• Prevent unrolling and preserve tissue integrity

Keywords

• Intestinal murine tissue
• Swiss-rolling technique
• Ex vivo method
• Histological tissue preparation