Evaluation of the Effects of Aquatic and Alcoholic Extracts of Thyfa Pollen on TNFα, TGFβ, Col-1 and VEGF Genes Expression in the Process of Third-Degree Burns Wound Healing

Abstract

Background and Aim: Burn injuries are usually defined as skin lesions caused by heat, temperature, electricity, chemicals, and radiation. Burn mortality is high in low income countries. The Typha is considered as a traditional medicine for a wound healing in Iran. This study aimed to investigate the molecular effects of aquatic and alcoholic extracts of Thyfa pollen on wound healing in Full -thickness (Third-degree burns) burns in rats. Material and Methods. The burn was induced by boiling water at 100° and a metallic ring in Sprague Dawley rats and then 5% ointment is prepared from aquatic and alcoholic extracts of Thyfa pollen. The extracts were applied to the damaged areas for 21 days after burn induction. Then, the expression of the mRNA of the TNF-α, TGFβ, Col-1, and VEGF genes were assessed by Real time-PCR technique. Results: Aquatic and alcoholic extracts of Thyfa pollen were identified to mainly contain Vitamin C and Catechin using HPLC method. Macroscopic studies and wound size analysis showed a reduction in wound size after 21 days (P<0.05). In addition, a significant increase in mRNA expression of TGFβ, Col-1 and VEGF genes was observed in the treated groups compared to the control group(P<0.001). Also, a significant reduction in mRNA expression of the TNF-α was indicated in the treated groups compared to the control group(P<0.001) Conclusion: The results of this study, indicated the aquatic and alcoholic extracts of Thyfa pollen have a potent wound-healing effect against burn injury in rats, therefore aquatic and, alcoholic extracts of Thyfa pollen can be noticed as a potential therapeutic in the future research of Third-degree burns.

Keywords

• third-degree burns
• typha
• tnf-α
• tgf-β
• vegf
• collagen-1