A comparative study on the effect of ethanol extract of wild Scrophularia deserti and streptomycin on Brucellla melitensis

Abstract

Introduction: Brucellosis or Malta fever is one of the most prevalent bacterial zoonosis which is transmitted to human being from animals. High fever, myalgia, and arthralgia of the large joints are the important symptoms. This study was aimed to evaluate the anti-brucellosis effect of ethanol extract of wild snapdragon on Brucella melitensis bacteria. Methods: This is an experimental in which after preparing the ethanol extract of wild Snapdragon (Scrophularia deserti), the anti-Brucellosis impressions of the extract on Brucella melitensis which is separated from goat’s abortive fetus were studied by serial dilution and disc diffusion method. Results: In disc diffusion test, the disc containing 60 μg/ml extract the inhibition zone was 9.7mm after 72, for streptomycin (20 μg/disc) 13.3 mm and for streptomycin plus 60 μg/disc extract discs it was 21.5 mm, after 72 hours incubation at 37 centigrade. After analyzing the data; the MIC for serial dilution test was 52% (576 μg/ml) for the first repetition and 50% (576 μg/ml) for the third repetition of the extract with OD600=1.56. For standard medicine "streptomycin" with OD600=1.67 it was 52% (360 μg/ml). Conclusion: Ethanol extract of wild Scrophularia deserti is effective on Brucellla melitensis and its consumption might be useful in these patients.

Keywords

• Minimum Inhibition Concentration(MIC)
• Ethanol extract
• Wild