Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging

Abstract

Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3‐fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state‐of‐the‐art commercial laser scanning microscope systems.

Keywords

• quantum dot triexciton imaging
• Airyscan
• STELLARIS
• 3D confocal imaging
• nanoscopy
• super‐resolution fluorescence imaging