Accentuated Peripheral Blood NK Cytotoxicity Forms an Unfavorable Background for Embryo Implantation and Gestation
Boris Dons’koi,
Oksana Onyshchuk,
Iryna Kononenko,
Vira Sirenko,
Natalia Bodnar,
Andrii Serbyn,
Anzhela Kozachok,
Yulia Brovarska,
Dariia Osypchuk,
Yaroslava Anochko,
Viktor Chernychov
Affiliations
Boris Dons’koi
Laboratory of Immunology, State Institution (Institute of Pediatrics, Obstetrics and Gynecology NAMED AFTER ACADEMICIAN O. Lukyanova of the National Academy of Medical Sciences of Ukraine), 04050 Kyiv, Ukraine
Oksana Onyshchuk
Reproductive Medicine Clinic “DAHNO IRM”, 04050 Kyiv, Ukraine
Iryna Kononenko
Reproductive Medicine Clinic “DAHNO IRM”, 04050 Kyiv, Ukraine
Vira Sirenko
Reproductive Medicine Clinic “DAHNO IRM”, 04050 Kyiv, Ukraine
Natalia Bodnar
Reproductive Medicine Clinic “DAHNO IRM”, 04050 Kyiv, Ukraine
Andrii Serbyn
Reproductive Medicine Clinic “DAHNO IRM”, 04050 Kyiv, Ukraine
Anzhela Kozachok
Reproductive Medicine Clinic “DAHNO IRM”, 04050 Kyiv, Ukraine
Yulia Brovarska
Reproductive Medicine Clinic “DAHNO IRM”, 04050 Kyiv, Ukraine
Dariia Osypchuk
Laboratory of Immunology, State Institution (Institute of Pediatrics, Obstetrics and Gynecology NAMED AFTER ACADEMICIAN O. Lukyanova of the National Academy of Medical Sciences of Ukraine), 04050 Kyiv, Ukraine
Yaroslava Anochko
Laboratory of Immunology, State Institution (Institute of Pediatrics, Obstetrics and Gynecology NAMED AFTER ACADEMICIAN O. Lukyanova of the National Academy of Medical Sciences of Ukraine), 04050 Kyiv, Ukraine
Viktor Chernychov
Laboratory of Immunology, State Institution (Institute of Pediatrics, Obstetrics and Gynecology NAMED AFTER ACADEMICIAN O. Lukyanova of the National Academy of Medical Sciences of Ukraine), 04050 Kyiv, Ukraine
Problem Many studies have demonstrated the negative impact of high rates of NK cytotoxicity (NKc) on reproductive processes, but there is no agreement as to the appropriateness and feasibility of using the NKc for routine diagnostic in IVF patients. This study conducted a retrospective analysis of embryo transfer (ET) success rates and live birth rates (LBR) in patients with different NKc values. Method of study 1854 cycles of ET were selected and divided into three groups according to NKc levels, and randomized by anamnesis and age: normal (nNKc, n = 871), elevated (eNKc, n = 759), and reduced NKc (rNKc, n = 123). ET with donors’ embryos (n = 101) were analyzed separately. NKc-to-K562 was measured in PBMC (peripheral blood mononuclear cells) by flow cytometry before ET. The patients did not obtain any additional treatments. Results Patients with eNKc, in addition to having reduced clinical pregnancy rates (OR1.59, p p p Conclusions Our findings highlighted the negative impact of high levels of NK cytotoxicity on pregnancy outcomes.
