DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates

Roxanne Glazier; Joshua M. Brockman; Emily Bartle; Alexa L. Mattheyses; Olivier Destaing; Khalid Salaita

doi:10.1038/s41467-019-12304-4

Nature Communications (Oct 2019)

DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates

Roxanne Glazier,
Joshua M. Brockman,
Emily Bartle,
Alexa L. Mattheyses,
Olivier Destaing,
Khalid Salaita

Affiliations

Roxanne Glazier: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Joshua M. Brockman: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Emily Bartle: Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham
Alexa L. Mattheyses: Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham
Olivier Destaing: Institute for Advanced Biosciences, Centre de Recherche Université Grenoble Alpes, Inserm U 1209, CNRS UMR 5309
Khalid Salaita: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University

DOI: https://doi.org/10.1038/s41467-019-12304-4
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

Read online

Podosomes are protrusive structures that coordinate diverse functions related to cell invasion, migration, bone resorption and immune surveillance. Here the authors integrate DNA nanotechnology with FLIM-FRET to demonstrate that podosomes apply pN integrin tensile forces to sense and respond to substrate mechanics.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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