Revista da Sociedade Brasileira de Medicina Tropical ()

Larvicidal and adulticidal effects and ultrastructural changes of larvae midgut epithelium of Musca domestica (Diptera: Muscidae) fed with Bacillus thuringiensis var. kyushuensis

  • Lorrane de Andrade Pereira,
  • Vitor dos Santos Baia Ferreira,
  • Nahara de Souza Leite,
  • Sandra Maria de Oliveira Souza,
  • Margareth Maria de Carvalho Queiroz,
  • Suzana Côrte-Real,
  • Viviane Zahner

DOI
https://doi.org/10.1590/0037-8682-0135-2019
Journal volume & issue
Vol. 52

Abstract

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Abstract INTRODUCTION: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative. METHODS: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut. RESULTS: We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected. CONCLUSIONS: The serovar, Btk176 might be a potential biocontrol agent for houseflies.

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