Magnetic chitosan membrane as an effective analytical tool for adsorptive removal of creatinine from biological samples

Abstract

Creatinine, a final product of metabolism released in the blood and removed by kidneys. The improper functioning of kidneys causes its higher accumulation in blood. In this study, a facile strategy was designed for artificial kidneys by using magnetic chitosan membrane. The membrane was synthesized by phase conversion process. The synthesized membrane was characterized by fourier transform infrared spectroscopy, elemental diffraction spectroscopy, scanning electron microscopy, and thermogravimetric analysis. Different factors were optimized for creatinine removal by membrane such as concentrations of creatinine, pH, time, interfering species. The linear response was obtained in the concentration range from 10 to 70 µM of creatinine. The results showed the limit of detection 7.33 µM and the limit of quantification 22.82 µM at optimized conditions. The magnetic chitosan membrane facilitates the development of the ecofriendly and cost effective method for the removal of biological toxins from real samples.

Keywords

• Chitosan
• dialysis membrane
• creatinine
• haemodialysis
• artificial kidney
• biological fluid