Effects of plasma acid on rat uterine tissue <i>in vitro</i>

Abstract

The aim of the study was to evaluate the effect of plasma acid on the uterine tissue of laboratory animals in vitro.Materials and methods. Treatment of dimethyl sulfoxide – water solution and water for injections with a spark discharge in air resulted in a decrease in pH, which contributed to generation of plasma acid in the solutions. We incubated uterine tissues in vitro in plasma acid at room temperature for 30 minutes. The treated tissues were examined histologically and immunohistochemically.Results. We showed that plasma acid had pronounced biological activity. Immunohistochemistry was used to show that, depending on the type of a solution, plasma acid altered generation of nitrosative damage products (3-NT) and oxidative DNA damage (8-OHdG) and modulated the number of cells with high proliferative potential (including CD133+ cells) and production of vascular endothelial growth factor (VEGF). These effects contributed to the general cytotoxicity of plasma acid solutions.Conclusion. During 30-minute exposure in vitro, plasma acid prepared from the dimethyl sulfoxide (DMSO) – water mixture exhibits various biological effects in uterine tissue samples obtained from experimental animals. Plasma-treated water exerts cytotoxic effects associated with oxidative DNA damage and promotes induction of pro-angiogenic activity in the uterine tissue. Plasma-treated DMSO does not have a cytotoxic effect. It inhibits cell proliferation, reducing the population of CD133+ cells and VEGF production in the tissue.

Keywords

• ткани матки
• плазменная кислота
• цитотоксичность