Genome survey report of Thai ricefish (Oryzias minutillus) (Actinopterygii: Beloniformes)

Abstract

Thai ricefish (Oryzias minutillus) is the smallest species in the Oryzias genus, widely distributed throughout Thailand. They are important in the trophic level of food webs within freshwater ecosystems. However, knowledge about the molecular data of this fish is lacking. Therefore, in this study, we surveyed the genome data of Thai ricefish collected from Mae Hong Son Province, Northwestern Thailand. In the initial assembly, the total genome and mitochondrial genome of Thai ricefish were 824 Mb and 16,954 bp, respectively. The total sizes of contig and scaffold were 547.7 and 585.9 Mb, respectively. The total genome size per mitochondrial size was 0.049. Phylogenetic relationship of Thai ricefish and related species of Oryzias was constructed based on mitochondrial genome. The nucleotide similarity of all genes in the mitogenome of Thai ricefish was compared with the related species of Oryzias from nucleotide database of the National Center for Biotechnology Information. The results provide data that increase knowledge of molecular genetics and a basis for further work on fish in genus Oryzias.

Keywords

• thai medaka
• northwestern thailand
• genome size
• mitogenome