ImmunoFISH for Adherent Cultured Mammalian Cells

Francesca  Rossiello; Marzia  Fumagalli; Fabrizio di Fagagna

doi:10.21769/BioProtoc.999

Bio-Protocol (Dec 2013)

ImmunoFISH for Adherent Cultured Mammalian Cells

Francesca Rossiello,
Marzia Fumagalli,
Fabrizio di Fagagna

Affiliations

Francesca Rossiello: The FIRC Institute for Molecular Oncology, IFOM, Milan, Italy
Marzia Fumagalli: The FIRC Institute for Molecular Oncology, IFOM, Milan, Italy
Fabrizio di Fagagna: The FIRC Institute for Molecular Oncology, IFOM, Milan, Italy

DOI: https://doi.org/10.21769/BioProtoc.999
Journal volume & issue: Vol. 3, no. 24

Abstract

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This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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