QTL Mapping by Chromosome Segment Substitution Lines (CSSLs) Reveals Candidate Gene Controlling Leaf Sucrose Content in Soybean (<i>Glycine max</i> (L.) Merr.)

Yuheng Wu; Chenyu He; Changheng Sun; Xiangran Wang; Zhaoming Qi; Qingshan Chen; Mingzhe Zhao; Xindong Yao; Dayong Zhang

doi:10.3390/agronomy13061592

Agronomy (Jun 2023)

QTL Mapping by Chromosome Segment Substitution Lines (CSSLs) Reveals Candidate Gene Controlling Leaf Sucrose Content in Soybean (<i>Glycine max</i> (L.) Merr.)

Yuheng Wu,
Chenyu He,
Changheng Sun,
Xiangran Wang,
Zhaoming Qi,
Qingshan Chen,
Mingzhe Zhao,
Xindong Yao,
Dayong Zhang

Affiliations

Yuheng Wu: College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Chenyu He: College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Changheng Sun: College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Xiangran Wang: Jiamusi Branch Institute, Heilongjiang Academy of Agricultural Sciences, Jiamusi 154000, China
Zhaoming Qi: College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Qingshan Chen: College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Mingzhe Zhao: Agricultural College, Shenyang Agricultural University, Shenyang 110866, China
Xindong Yao: College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Dayong Zhang: College of Agriculture, Northeast Agricultural University, Harbin 150030, China

DOI: https://doi.org/10.3390/agronomy13061592
Journal volume & issue: Vol. 13, no. 6
p. 1592

Abstract

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Understanding the genetic basis of leaf sucrose content can provide a novel way in improving soybean yields. To identify the related QTLs, 190 materials of chromosome fragment substitution lines (CSSLs) were used in this study. The CSSLs were developed from the cross between the cultivated soybean Suinong 14 (SN14) and wild soybean ZYD00006. Only one QTL with a high logarithm of odds (LOD) score was detected in 2021 and 2022 among 3780 bin markers (combined by 580,524 SNPs) distributed in 20 chromosomes. Nine candidate genes were screened and Glyma.14G029100 was considered as the hub gene. A promoter difference and CDS mutant was found among the parents and the reference genome, which lead to the relative transcriptional level difference.. Our results lay the groundwork for further research into its genetic mechanism.

Published in Agronomy

ISSN: 2073-4395 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture
Website: http://www.mdpi.com/journal/agronomy

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