Regional Diversity in the Postsynaptic Proteome of the Mouse Brain
Marcia Roy,
Oksana Sorokina,
Colin McLean,
Silvia Tapia-González,
Javier DeFelipe,
J. Douglas Armstrong,
Seth G. N. Grant
Affiliations
Marcia Roy
Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh EH16 4SB, UK
Oksana Sorokina
School of Informatics, University of Edinburgh, Edinburgh EH8 9AB, UK
Colin McLean
School of Informatics, University of Edinburgh, Edinburgh EH8 9AB, UK
Silvia Tapia-González
Departamento de Neurobiología Funcional y de Sistemas, Instituto Cajal (CSIC), Ave. Doctor Arce 37, 28002 Madrid and Laboratorio Cajal de Circuitos Corticales, Centro de Tecnología Biomédica (UPM), 28223 Pozuelo de Alarcón, Madrid, Spain
Javier DeFelipe
Departamento de Neurobiología Funcional y de Sistemas, Instituto Cajal (CSIC), Ave. Doctor Arce 37, 28002 Madrid and Laboratorio Cajal de Circuitos Corticales, Centro de Tecnología Biomédica (UPM), 28223 Pozuelo de Alarcón, Madrid, Spain
J. Douglas Armstrong
School of Informatics, University of Edinburgh, Edinburgh EH8 9AB, UK
Seth G. N. Grant
Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh EH16 4SB, UK
The proteome of the postsynaptic terminal of excitatory synapses comprises over one thousand proteins in vertebrate species and plays a central role in behavior and brain disease. The brain is organized into anatomically distinct regions and whether the synapse proteome differs across these regions is poorly understood. Postsynaptic proteomes were isolated from seven forebrain and hindbrain regions in mice and their composition determined using proteomic mass spectrometry. Seventy-four percent of proteins showed differential expression and each region displayed a unique compositional signature. These signatures correlated with the anatomical divisions of the brain and their embryological origins. Biochemical pathways controlling plasticity and disease, protein interaction networks and individual proteins involved with cognition all showed differential regional expression. Combining proteomic and connectomic data shows that interconnected regions have specific proteome signatures. Diversity in synapse proteome composition is key feature of mouse and human brain structure.
