Functional genome Annotation in Bacterial infections: A Review

Abstract

Functional annotation provides the information of gene about its biological identity and various types of molecular function and biological role like sub-cellular location and its expression. It functions by attaching biological information of gene and protein. The primary level of annotation is performed by using Basic logical Alignment Tool (BLAST) for finding similarity and then annotation of genes are performed based on this information. The function of gene and variation in gene functioning can be understand by the elucidation of genome annotation. Ensemble annotation tool gives fast computational annotation in eukaryotes derived from mRNA, RNAseq. The two groups HAVANA and RefSeq (human and vertebrate analysis and annotation and Reference Sequence respectively) give rise to wide genome manual transcript annotation and are the finest computational method which recognize 70% of manual annotated loci. The prokaryotic genomes are involved in various genome projects with utilization of Next Generation Sequencing (NGS) it leads to decrease in time and money consumption. Bacterial genome annotation is done by the help of annotation pipeline it provides NCBI resource of content including Nucleotide, Protein, BLAST by the use of software GLIMMER which helps in identification of long protein coding gene mainly in bacteria. A novel bacteriophage Abp2 infects the bacteria Acinetobacter baumannii a multi drug resistance (MDR). The genome annotation of this phage reveals that it is a circular DNA with 85 open reading frame (ORF) with putative genes in which 41 known function and 47 unknown function protein were recognized. This review article summarizes genome annotation function and structural aspects.

Keywords

• bacteria
• gene
• dna
• elucidation
• genome annotation
• mrna