Identification of four animal derived components in milk and dairy products

Abstract

ObjectiveTo detect the adulteration in milk and dairy products， the method for identification of animal derived components in milk and dairy products was constructed.MethodsThe specific primer probes of cattle， sheep， donkey and buffalo were designed and synthesized. The nucleic acid extraction method in milk and dairy products was optimized. The specificity， sensitivity and applicability of the designed fluorescent PCR method were verified.ResultsThe method had strong specificity and sensitivity for common animal species. The detection sensitivities of cattle， sheep， donkey and buffalo were 0.001， 0.01， 0.01 and 0.01 ng， respectively.ConclusionThe specificity， sensitivity， detection limit and other indicators of the constructed method can meet the needs of daily experiments， and provide strong technical support for supervision and testing.

Keywords

• milk and dairy products
• animal derived components
• specificity
• applicability