IMC-Denoise: a content aware denoising pipeline to enhance Imaging Mass Cytometry

Peng Lu; Karolyn A. Oetjen; Diane E. Bender; Marianna B. Ruzinova; Daniel A. C. Fisher; Kevin G. Shim; Russell K. Pachynski; W. Nathaniel Brennen; Stephen T. Oh; Daniel C. Link; Daniel L. J. Thorek

doi:10.1038/s41467-023-37123-6

Nature Communications (Mar 2023)

IMC-Denoise: a content aware denoising pipeline to enhance Imaging Mass Cytometry

Peng Lu,
Karolyn A. Oetjen,
Diane E. Bender,
Marianna B. Ruzinova,
Daniel A. C. Fisher,
Kevin G. Shim,
Russell K. Pachynski,
W. Nathaniel Brennen,
Stephen T. Oh,
Daniel C. Link,
Daniel L. J. Thorek

Affiliations

Peng Lu: Department of Biomedical Engineering, Washington University in St. Louis
Karolyn A. Oetjen: Department of Medicine, Washington University School of Medicine
Diane E. Bender: The Bursky Center for Human Immunology and Immunotherapy Programs Immunomonitoring Laboratory, Washington University School of Medicine
Marianna B. Ruzinova: Department of Pathology & Immunology, Washington University School of Medicine
Daniel A. C. Fisher: Department of Medicine, Washington University School of Medicine
Kevin G. Shim: Department of Medicine, Washington University School of Medicine
Russell K. Pachynski: Department of Medicine, Washington University School of Medicine
W. Nathaniel Brennen: Department of Oncology, Sidney Kimmel Comprehensive Cancer Center (SKCCC), Johns Hopkins University
Stephen T. Oh: Department of Medicine, Washington University School of Medicine
Daniel C. Link: Department of Medicine, Washington University School of Medicine
Daniel L. J. Thorek: Department of Biomedical Engineering, Washington University in St. Louis

DOI: https://doi.org/10.1038/s41467-023-37123-6
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 16

Abstract

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Multiplexed imaging technologies can reveal the complex cellular and molecular profiles of tissue. Here, the authors develop and implement a denoising pipeline to significantly enhance imaging mass cytometry quality and improve single-cell analyses.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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