Nature Communications (Apr 2022)
A functional map of HIV-host interactions in primary human T cells
- Joseph Hiatt,
- Judd F. Hultquist,
- Michael J. McGregor,
- Mehdi Bouhaddou,
- Ryan T. Leenay,
- Lacy M. Simons,
- Janet M. Young,
- Paige Haas,
- Theodore L. Roth,
- Victoria Tobin,
- Jason A. Wojcechowskyj,
- Jonathan M. Woo,
- Ujjwal Rathore,
- Devin A. Cavero,
- Eric Shifrut,
- Thong T. Nguyen,
- Kelsey M. Haas,
- Harmit S. Malik,
- Jennifer A. Doudna,
- Andrew P. May,
- Alexander Marson,
- Nevan J. Krogan
Affiliations
- Joseph Hiatt
- Department of Microbiology and Immunology, University of California
- Judd F. Hultquist
- Division of Infectious Diseases, Northwestern University Feinberg School of Medicine
- Michael J. McGregor
- Gladstone Institutes
- Mehdi Bouhaddou
- Gladstone Institutes
- Ryan T. Leenay
- Chan Zuckerberg BioHub
- Lacy M. Simons
- Division of Infectious Diseases, Northwestern University Feinberg School of Medicine
- Janet M. Young
- Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center
- Paige Haas
- Gladstone Institutes
- Theodore L. Roth
- Department of Microbiology and Immunology, University of California
- Victoria Tobin
- Department of Microbiology and Immunology, University of California
- Jason A. Wojcechowskyj
- Gladstone Institutes
- Jonathan M. Woo
- Department of Microbiology and Immunology, University of California
- Ujjwal Rathore
- Department of Microbiology and Immunology, University of California
- Devin A. Cavero
- Department of Microbiology and Immunology, University of California
- Eric Shifrut
- Department of Microbiology and Immunology, University of California
- Thong T. Nguyen
- Gladstone Institutes
- Kelsey M. Haas
- Gladstone Institutes
- Harmit S. Malik
- Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center
- Jennifer A. Doudna
- Gladstone Institutes
- Andrew P. May
- Chan Zuckerberg BioHub
- Alexander Marson
- Department of Microbiology and Immunology, University of California
- Nevan J. Krogan
- Gladstone Institutes
- DOI
- https://doi.org/10.1038/s41467-022-29346-w
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 15
Abstract
Here, Hiatt et al. report the knock-out of over 400 genes in primary CD4+ T cells to assess their functional role in HIV replication, finding 86 initial candidates of which 47 are validated as HIV host factors, including 23 with restrictive activity.
