Transcriptome Analysis of the Effect of Acute Ammonia Stress on <i>Pseudobagrus ussuriensis</i> Liver Tissue

Abstract

Excess ammonia can damage the growth and development of fish. Pseudobagrus ussuriensis is a scaleless fish with important economic value that is more sensitive to ammonia stress. In this study, P. ussuriensis was explored using different ammonia concentrations [control (0 mg/L), CL; low stress (10 mg/L), T1L; and high stress (50 mg/L), T2L] for 48 h. Compared to the control group, the liver cells in the T1L group showed slight damage, while the T2L group was severely damaged, with the cells being loosely arranged, with nuclei lysis and cell vacuolization. The activities of superoxide dismutase, catalase, and glutathione in the T1L and T2L groups were significantly lower than those in the CL group (p P. ussuriensis, causing genetic mutations and cellular carcinogenesis, thereby accelerating cell apoptosis.

Keywords

• <i>Pseudobagrus ussuriensis</i>
• acute ammonia stress
• liver tissue
• transcriptome characteristics