Potential Anti-aging Components From Moringa oleifera Leaves Explored by Affinity Ultrafiltration With Multiple Drug Targets

Yongbing Xu; Yongbing Xu; Yongbing Xu; Yongbing Xu; Guilin Chen; Guilin Chen; Guilin Chen; Guilin Chen; Mingquan Guo; Mingquan Guo; Mingquan Guo; Mingquan Guo

doi:10.3389/fnut.2022.854882

Frontiers in Nutrition (May 2022)

Potential Anti-aging Components From Moringa oleifera Leaves Explored by Affinity Ultrafiltration With Multiple Drug Targets

Yongbing Xu,
Yongbing Xu,
Yongbing Xu,
Yongbing Xu,
Guilin Chen,
Guilin Chen,
Guilin Chen,
Guilin Chen,
Mingquan Guo,
Mingquan Guo,
Mingquan Guo,
Mingquan Guo

Affiliations

Yongbing Xu: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China
Yongbing Xu: College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
Yongbing Xu: Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, China
Yongbing Xu: Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai, China
Guilin Chen: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China
Guilin Chen: College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
Guilin Chen: Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, China
Guilin Chen: Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai, China
Mingquan Guo: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China
Mingquan Guo: College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
Mingquan Guo: Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, China
Mingquan Guo: Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai, China

DOI: https://doi.org/10.3389/fnut.2022.854882
Journal volume & issue: Vol. 9

Abstract

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Moringa oleifera (M. oleifera), widely used in tropical and subtropical regions, has been reported to possess good anti-aging benefits on skincare. However, the potential bioactive components responsible for its anti-aging effects, including anti-collagenase, anti-elastase, and anti-hyaluronidase activities, have not been clarified so far. In this study, M. oleifera leaf extracts were first conducted for anti-elastase and anti-collagenase activities in vitro by spectrophotometric and fluorometric assays, and the results revealed that they possessed good activities against skin aging-related enzymes. Then, multi-target bio-affinity ultrafiltration coupled to high-performance liquid chromatography-mass spectrometry (AUF-HPLC-MS) was applied to quickly screen anti-elastase, anti-collagenase, and anti-hyaluronidase ligands in M. oleifera leaf extracts. Meanwhile, 10, 8, and 14 phytochemicals were screened out as the potential anti-elastase, anti-collagenase, and anti-hyaluronidase ligands, respectively. Further confirmation of these potential bioactive components with anti-aging target enzymes was also implemented by molecule docking analysis. In conclusion, these results suggest that the M. oleifera leaves might be a very promising natural source of anti-aging agent for skincare, which can be further explored in the cosmetics and cosmeceutical industries combating aging and skin wrinkling.

Published in Frontiers in Nutrition

ISSN: 2296-861X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.frontiersin.org/journals/nutrition/

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