Shoot Regeneration from Leaf Explants of Withania somnifera (L.) Dunal

Abstract

Regeneration from leaf explants of Withania somnifera (L.) for mass propagation was studied on Murashige and Skoog�s medium supplemented with Kinetin (Kn) and 6-benzylaminopurine (BAP) alone or in combination. Shoot buds were induced from the midrib on the abaxial side in presence of Kn and BAP (4 �M). These shoot buds developed into shoots on the same medium. Rooting of these shoots was achieved in 0.5 �M of IBA.