Measurement of Michaelis Constants for ATP and Mg2+ in Bioluminescence Reaction of Luciferase by a Home-Made Luminometer

Abstract

Effects of ATP and Mg2+ concentrations on bioluminescence reaction of luciferase (Photinus pyralis) were investigated by home-made luminometer. The Michaelis constants of the enzyme for ATP and Mg2+ obtained from the Lineweaver-Burk graph, were 61.9 mM ±3.3 mM and 251.6mM ± 39.0mM respectively. Optimum concentration of Mg2+ for maximum luminescence was 0.004 M.

Keywords

• michaelis constant
• luciferase
• atp
• photinus pyralis
• american firefly
• luminometer
• bioluminescence