The Effect of Resistance Training on Some Markers of Apoptosis Caused by Diabetic Cardiomyopathy and Morphine withdrawal Syndrome in Diabetic Male Rats

Abstract

Introduction: Morphine withdrawal syndrome is one of the aggravating factors of apoptosis in diabetic cardiomyopathy. The purpose of this research was to investigate the effect of resistance training on some markers of apoptosis caused by diabetic cardiomyopathy and morphine withdrawal syndrome in diabetic male rats. Materials and Methods: In this experimental study, 32 male wistar rats were randomly divided into 4 groups with 8 rats, including diabetes, morphine diabetes, diabetes + resistance training, diabetes morphine + resistance training. After implementing the diabetes induction protocol, the addicted groups received morphine orally for 21 days, and then the training groups participated in the resistance training protocol for 8 weeks. Then all the rats were killed and dissected and their heart tissue was removed. ELISA kits were used to evaluate apoptotic factors. Results: In diabetic rats with withdrawal syndrome, BAX values and BAX/BCL2 ratio increased significantly and BCL2 value decreased compared to the diabetic group. Resistance training in diabetic rats with withdrawal syndrome caused a significant increase in BCL2 and a decrease in the amount of BAX and BAX/BCL2 ratio compared to the diabetic group with withdrawal syndrome. Also, resistance training in the diabetic group increased BCL2 and decreased BAX and the BAX/BCL2 ratio compared to the diabetic control group. In none of the cases, there was no significant difference between the diabetes-withdrawal syndrome-resistance training group and the diabetic control group. Conclusion: The results of the present study showed that resistance training reduces the apoptotic factors of heart tissue caused by morphine withdrawal syndrome in diabetic rats.

Keywords

• diabetes mellitus
• morphine
• resistance training