Protocol for the integration of fiber photometry and social behavior in rodent models

Dylan J. Terstege; Matthew Dawson; Naila F. Jamani; Mio Tsutsui; Jonathan R. Epp; Derya Sargin

doi:10.1016/j.xpro.2023.102689

STAR Protocols (Dec 2023)

Protocol for the integration of fiber photometry and social behavior in rodent models

Dylan J. Terstege,
Matthew Dawson,
Naila F. Jamani,
Mio Tsutsui,
Jonathan R. Epp,
Derya Sargin

Affiliations

Dylan J. Terstege: Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada; Corresponding author
Matthew Dawson: Department of Psychology, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada; Alberta Children’s Hospital Research Institute, University of Calgary, Calgary, AB T2N 1N4, Canada
Naila F. Jamani: Department of Psychology, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada; Alberta Children’s Hospital Research Institute, University of Calgary, Calgary, AB T2N 1N4, Canada
Mio Tsutsui: Department of Psychology, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada; Alberta Children’s Hospital Research Institute, University of Calgary, Calgary, AB T2N 1N4, Canada
Jonathan R. Epp: Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada
Derya Sargin: Department of Psychology, University of Calgary, Calgary, AB T2N 1N4, Canada; Department of Physiology and Pharmacology, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada; Alberta Children’s Hospital Research Institute, University of Calgary, Calgary, AB T2N 1N4, Canada; Corresponding author

DOI: https://doi.org/10.1016/j.xpro.2023.102689
Journal volume & issue: Vol. 4, no. 4
p. 102689

Abstract

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Summary: Fiber photometry offers insight into cell-type-specific activity underlying social interactions. We provide a protocol for the integration of fiber photometry recordings into the analysis of social behavior in rodent models. This includes considerations during surgery, notes on synchronizing fiber photometry with behavioral recordings, advice on using multi-animal behavioral tracking software, and scripts for the analysis of fiber photometry recordings.For complete details on the use and execution of this protocol, please refer to Dawson et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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