Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer

Laura Rodriguez de la Fuente; Andrew M.K. Law; David Gallego-Ortega; Fatima Valdes-Mora

STAR Protocols (Dec 2021)

Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer

Laura Rodriguez de la Fuente,
Andrew M.K. Law,
David Gallego-Ortega,
Fatima Valdes-Mora

Affiliations

Laura Rodriguez de la Fuente: Tumour Development Group, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia; St. Vincent’s Clinical School, Faculty of Medicine, University of New South Wales Sydney, Sydney, NSW 2010, Australia; Cancer Epigenetic Biology and Therapeutics Lab, Children’s Cancer Institute, Lowy Cancer Research Centre, University of New South Wales, Sydney, NSW 2052, Australia
Andrew M.K. Law: Tumour Development Group, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia; St. Vincent’s Clinical School, Faculty of Medicine, University of New South Wales Sydney, Sydney, NSW 2010, Australia
David Gallego-Ortega: Tumour Development Group, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia; St. Vincent’s Clinical School, Faculty of Medicine, University of New South Wales Sydney, Sydney, NSW 2010, Australia; School of Biomedical Engineering, Faculty of Engineering and IT, University of Technology Sydney, Ultimo, NSW 2007 Australia; Corresponding author
Fatima Valdes-Mora: Cancer Epigenetic Biology and Therapeutics Lab, Children’s Cancer Institute, Lowy Cancer Research Centre, University of New South Wales, Sydney, NSW 2052, Australia; School of Women’s and Children’s Health, Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100841

Abstract

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Summary: Cell preparation with a high rate of viable cells is required to obtain reliable single-cell transcriptomic and epigenomic data. This protocol describes a technique for digestion and single-cell isolation from mouse mammary tumors to achieve ∼90% of viable cells, which can be subsequently processed in a diverse array of high-throughput single-cell “omic platforms,” both in an unbiased manner or after selection of a specific cell population.For complete details on the use and execution of this protocol, please refer to Valdes-Mora et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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