RFLP-ANALYSIS OF BRADYRHIZOBIUM JAPONICUM NODULE BACTERIA, WIDESPREAD IN AGROCENOSIS OF UKRAINE

Abstract

Aim. To investigate the genetic diversity of soybean nodule bacteria, widespread in agrocenoses of Ukraine. Methods. Nodule bacteria were isolated from soybean nodules grown in soil samples selected in different regions of Ukraine. The amplification of the intergenic region 16S-23S rRNA (ITS-region) was conducted with the use of primers FGPS1490-72 and FGPL132-38. For PCR-products restriction there were applied restriction endonucleases MspI, HaeIII and NdeII. Results. The diversity of Bradyrhizobium japonicum strains with different growthrates was investigated using PCR-RFLP method. According to analysis of 16S-23S rDNA with enzymes MspI, HaeIII and NdeII it was obtained that all the intensivegrowing strains had the same restriction profiles, so they were combined into a single genetic group. Based on ITS-region restriction the slow-growing strains were assigned into two genetic groups using MspI enzyme, three genetic groups using HaeIII and four genomogroups using NdeII enzyme. Сonclusions. High heterogeneity of B. japonicum strains allocated from soybean agrocenoses was found. They belong to different ITS-types according to the structure of intergenic region. Slow-growing strains used to be more heterogeneous, in comparison with more intensive-growth strains, which formed a homogeneous genomogroup.

Keywords

• bradyrhizobium japonicum
• генетичний поліморфізм
• iтs-регіон
• rflp-аналіз
• соя