Single-Molecule Long-Read Sequencing of <i>Zanthoxylum bungeanum</i> Maxim. Transcriptome: Identification of Aroma-Related Genes

Abstract

Zanthoxylum bungeanum Maxim. is an economically important tree species that is resistant to drought and infertility, and has potential medicinal and edible value. However, comprehensive genomic data are not yet available for this species, limiting its potential utility for medicinal use, breeding programs, and cultivation. Transcriptome sequencing provides an effective approach to remedying this shortcoming. Herein, single-molecule long-read sequencing and next-generation sequencing approaches were used in parallel to obtain transcript isoform structure and gene functional information in Z. bungeanum. In total, 282,101 reads of inserts (ROIs) were identified, including 134,074 full-length non-chimeric reads, among which 65,711 open reading frames (ORFs), 50,135 simple sequence repeats (SSRs), and 1492 long non-coding RNAs (lncRNAs) were detected. Functional annotation revealed metabolic pathways related to aroma components and color characteristics in Z. bungeanum. Unexpectedly, 30 transcripts were annotated as genes involved in regulating the pathogenesis of breast and colorectal cancers. This work provides a comprehensive transcriptome resource for Z. bungeanum, and lays a foundation for the further investigation and utilization of Zanthoxylum resources.

Keywords

• aroma metabolic pathway
• long non-coding RNA
• single-molecule real-time sequencing
• <i>Zanthoxylum bungeanum</i> Maxim.