High expression of PDCD11 in colorectal cancer and its correlation with the prognosis and immune cell infiltration

Xiongfeng Li; Gaowa Sharen; Minjie Zhang; Lei Zhang; Kejian Liu; Yu Wang; Haidong Cheng; Mingxing Hou

Heliyon (Aug 2024)

High expression of PDCD11 in colorectal cancer and its correlation with the prognosis and immune cell infiltration

Xiongfeng Li,
Gaowa Sharen,
Minjie Zhang,
Lei Zhang,
Kejian Liu,
Yu Wang,
Haidong Cheng,
Mingxing Hou

Affiliations

Xiongfeng Li: Department of Gastrointestinal Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010059, Inner Mongolia, China
Gaowa Sharen: Department of Pathology, Affiliated Hospital of Inner Mongolia Medical University & Department of Pathological Anatomy, College of Basic Medicine of Inner Mongolia Medical University, Hohhot, 010059, Inner Mongolia, China
Minjie Zhang: Department of Ultrasound, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010059, Inner Mongolia, China
Lei Zhang: Department of Gastrointestinal Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010059, Inner Mongolia, China
Kejian Liu: Department of Gastrointestinal Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010059, Inner Mongolia, China
Yu Wang: Department of Gastrointestinal Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010059, Inner Mongolia, China
Haidong Cheng: Department of Gastrointestinal Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010059, Inner Mongolia, China; Corresponding author.Department of Gastrointestinal Surgery, Affiliated Hospital of Inner Mongolia Medical University, No. 1 Tongdao North Road, Hohhot, 010059, Inner Mongolia, China.
Mingxing Hou: Department of Gastrointestinal Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010059, Inner Mongolia, China; Corresponding author. Department of Gastrointestinal Surgery, Affiliated Hospital of Inner Mongolia Medical University, No. 1 Tongdao North Road, Hohhot, 010059, Inner Mongolia, China.

Journal volume & issue: Vol. 10, no. 15
p. e35002

Abstract

Read online

Objective: To undertake a comprehensive assay of PDCD11 expression in colorectal cancer (CRC) and its association with prognosis and immune cell infiltration (ICIN) utilizing bioinformatics tools. Methods: The PDCD11 expression in CRC and pan-cancer was quantified through datasets from TCGA and GEO databases, and the assay was conducted through R software and the GEPIA database. Moreover, mRNA and protein expression data of PDCD11 were attained from the HPA database. It was attempted to establish protein-protein interaction networks of PDCD11 via the STRING and GeneMANIA databases. The association of PDCD11 expression with CRC staging was evaluated through R software, while its association with CRC and pan-cancer prognosis was figured out via the GEPIA database. Furthermore, the relationship of PDCD11 expression with ICIN was assayed using R software and the TIMER database. Additionally, the influences of PDCD11 knockdown on the proliferation, apoptosis, and migration of colon cancer RKO cell lines was evaluated. Results: PDCD11 exhibited elevated expression in CRC and various other malignancies, potentially indicating a promotive role in cancer progression. Overexpression of PDCD11 was found to correlate with attenuated overall survival in CRC and other malignancies. Moreover, PDCD11 demonstrated promising predictive capabilities for distinguishing between tumor and non-tumor tissues. The positive association of high PDCD11 expression with the infiltration of neutrophils, dendritic cells, CD8+ T cells, CD4+ T cells, and macrophages, as well as with the expression of immune checkpoint molecules CTLA4 and PD-1 was noteworthy. Lentivirus-mediated PDCD11 knockdown suppressed RKO cell proliferation, colony formation, and migration, while triggered apoptosis in these cells. Conclusion: The outcomes unveiled the noticeable function of PDCD11 in CRC and various other malignancies, emphasizing its potential as a prognostic biomarker and therapeutic target.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

About the journal

Abstract

Keywords