Arquivo Brasileiro de Medicina Veterinária e Zootecnia (Apr 2008)

Fator do plasma seminal associado à integridade de membrana de espermatozóides suínos pós-descongelamento Seminal plasma factor associated to post-thawing swine sperm membrane integrity

  • I. Bianchi,
  • T. Collares,
  • V.F. Campos,
  • P.V. Cavalcanti,
  • C. Kaefer,
  • E.K. Corrêa,
  • O.A. Dellagostin,
  • T. Lucia Jr.,
  • J.C. Deschamps,
  • M.N. Corrêa

DOI
https://doi.org/10.1590/S0102-09352008000200017
Journal volume & issue
Vol. 60, no. 2
pp. 384 – 388

Abstract

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Neste estudo, identificaram-se polipeptídeos associados à integridade da membrana plasmática (IMP) de espermatozóides suínos após o processo de congelamento/descongelamento. Por meio do perfil protéico do plasma seminal em SDS-PAGE, observou-se a presença de nove bandas polipeptídicas com pesos moleculares que variaram de 11,97 a 122,52kDa. Detectou-se que uma banda de 26,58kDa esteve associada à baixa IMP (Polypeptides associate to membrane integrity (MI) of swine spermatozoa submitted to freezing and thawing were identified. The protein profile of seminal plasma analyzed by SDS-PAGE allowed the identification of nine polypeptide bands with molecular weight ranging from 11.97 to 122.52kDa. One 26.58kDa band was associated with reduced MI (<55%). No associations among other bands and MI were observed. The 26.58kDa factor is associated with reduction of membrane integrity of swine spermatozoa after freezing and thawing.

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