Rutin Prevents Dexamethasone-Induced Muscle Loss in C2C12 Myotube and Mouse Model by Controlling FOXO3-Dependent Signaling
Young-Sool Hah,
Won Keong Lee,
Seung-Jun Lee,
Sang Yeob Lee,
Jin-Hee Seo,
Eun Ji Kim,
Yeong-In Choe,
Sang Gon Kim,
Jun-Il Yoo
Affiliations
Young-Sool Hah
Department of Orthopedics, Institute of Health Sciences, Gyeongsang National University School of Medicine and Hospital, Jinju 52727, Republic of Korea
Won Keong Lee
Biomedical Research Institute, Gyeongsang National University Hospital, Jinju 52727, Republic of Korea
Seung-Jun Lee
Department of Convergence of Medical Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea
Sang Yeob Lee
Biomedical Research Institute, Gyeongsang National University Hospital, Jinju 52727, Republic of Korea
Jin-Hee Seo
Crop Production Technology Research Division, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of Korea
Eun Ji Kim
Department of Orthopedics, Institute of Health Sciences, Gyeongsang National University School of Medicine and Hospital, Jinju 52727, Republic of Korea
Yeong-In Choe
Department of Orthopedics, Institute of Health Sciences, Gyeongsang National University School of Medicine and Hospital, Jinju 52727, Republic of Korea
Sang Gon Kim
Anti-Aging Research Group, Gyeongnam Oriental Anti-Aging Institute, Sancheong 52215, Republic of Korea
Jun-Il Yoo
Department of Orthopedic Surgery, Inha University Hospital, Incheon 22332, Republic of Korea
One of the causes of sarcopenia is that homeostasis between anabolism and catabolism breaks down due to muscle metabolism changes. Rutin has shown antioxidant and anti-inflammatory effects in various diseases, but there are few studies on the effect on muscle loss with aging. The effect of rutin on muscle loss was evaluated using dexamethasone-induced muscle loss C2C12 myoblast and mouse model. In the group treated with dexamethasone, the muscle weight of gastrocnemius (GA), tibialis anterior (TA), and extensor digitorum longus (EDL) in the mouse model were significantly decreased (p p p p p p p p p p p p p p p p < 0.001 in FOXO3) when rutin was treated. The present study shows that rutin blocks the FOXO3/MAFbx and FOXO3/MuRf1 pathways to prevent protein catabolism. Therefore, rutin could be a potential agent for muscle loss such as sarcopenia through the blocking ubiquitin-proteasome pathway associated with catabolic protein degradation.
