Activity of matrix metalloproteinases and their tissue inhibitors in serum in chronic granulating periodontitis

Abstract

Aim. The aim of the work is to study the activity of matrix metalloproteinases and their tissue inhibitors in chronic granular periodontitis. Materials and methods. We examined serum of 15 patients with chronic granulating periodontitis and 15 healthy male blood donors aged 32 to 37 years. Determination of MMP-8, MMP-9 and TIMP-1 activity was performed by solid-phase enzyme immunoassay using test systems (R&D Diagnostics Inc., USA) on the automatic immunoenzyme complex “GBG Star Fax 2100” produced by “Awareness Technology Inc.” (USA). Statistical evaluation of the results was carried out using the generated database in the program Statistica v. 10.0 (StatSoft. Inc., USA), № STA999K347156-W. Results. It was established that in the phase of exacerbation of chronic granulating periodontitis in the blood serum of patients there was an increase in concentrations of MMP-8 and MMP-9, which exceeded the content of TIMP-1, which was accompanied by a decrease in the ratio of MMP-8/TIMP-1 and MMP-9/TIMP-1, compared with those for healthy individuals. In the phase of early convalescence, 3–5 days after treatment of patients with exacerbation of chronic granulating periodontitis, concentrations of MMP-8 and MMP-9 in the blood serum of the examined patients decreased more dynamically than the concentration of TIMP-1, that was accompanied by an increase in the ratio of MMP-8/TIMP-1 and MMP-9/TIMP-1, at the same time the full normalization of MMP-8, MMP-9, TIMP-1 and the ratio of MMP-8/TIMP-1 and MMP-9/TIMP-1 did not happen. Conclusions. Chronic granulating periodontitis is accompanied by changes in the blood serum of patients with MMP-8, MMP-9 and TIMP-1, which depend on the phase of the pathological process. The highest concentrations of MMP-8, MMP-9 and TIMP-1 are recorded in the acute phase of the disease. In the phase of early convalescence, 3–5 days after the remediation of the focus of inflammation, the concentrations of MMP-8, MMP-9 and TIMP-1 in serum are decreased, while the dynamics of decreasing of the content of MMP-8 and MMP-9 prevails over that for TIMP-1. The coefficients MMP-8/TIMP-1 and MMP-9/TIMP-1, which characterize the balance in the system of MMP/TIMP, allow assessing the state of the system in the dynamics of the disease.

Keywords

• chronic granulating periodontitis
• matrix metalloproteinases
• tissue inhibitors of matrix metalloproteinases