Ciência Animal Brasileira (Sep 2013)

IDENTIFICAÇÃO DE MAMÍFEROS SILVESTRES DO PANTANAL SUL-MATO-GROSSENSE PORTADORES DE Leptospira spp.

  • Vitor Rademaker,
  • Natalie Olifiers,
  • Rita de Cassia Bianchi,
  • Guilherme Miranda Mourão,
  • Flabio Ribeiro de Araújo,
  • Cleber Oliveira Soares,
  • Carina Elisei de Oliveira,
  • Rosielle Campozano Viana,
  • Zenaide Maria de Moaris,
  • Grácia Maria Soares Rosinha,
  • Silvio Arruda Vasconcellos,
  • Anahi Souto Vieira,
  • Fabiana Lopes Rocha,
  • Aiesca Oliveira Pellegrin

DOI
https://doi.org/10.5216/cab.v14i3.17147
Journal volume & issue
Vol. 14, no. 3
pp. 373 – 380

Abstract

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A survey of Leptospira spp. in wild mammals from the southern Pantanal of Mato Grosso do Sul was performed by microscopic agglutination test (MAT) and polymerase chain reaction (PCR). The serovars most frequently found were Hardjobovis (28%), Icterohemorhagiae (12%), M110/2006 strain (isolated from Cerdocyon thous, 16%), Canicola (L014 isolated from Bos Taurus, 4%), Whitcombi (4%), Pomona (20%), Autumnalis (12%) and Copenhageni (M9/99 isolated from Rattus norvegicus, 4%). From the 79 samples tested by PCR, 21 (26.58%) were positive, resulting in the amplification fragment of approximately 331pb. Two amplified fragments from C. thous were cloned, sequenced and identified as L. interrogans by phylogenetic analysis.

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