Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens
Ahmed Elbestawy,
Hany Ellakany,
Mahmoud Sedeik,
Ahmed Gado,
Mervat Abdel-Latif,
Ahmed Noreldin,
Ahmed Orabi,
Ismail Radwan,
Wafaa Abd El-Ghany
Affiliations
Ahmed Elbestawy
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt
Hany Ellakany
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt
Mahmoud Sedeik
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, Egypt
Ahmed Gado
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt
Mervat Abdel-Latif
Nutrition and Veterinary Clinical Nutrition Department, Faculty of Veterinary Medicine, Damanhour University, El-Beheira 22511, Egypt
Ahmed Noreldin
Department of Histology and Cytology, Faculty of Veterinary Medicine, Damanhour University, El-Beheira 22511, Egypt
Ahmed Orabi
Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt
Ismail Radwan
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 62511, Egypt
Wafaa Abd El-Ghany
Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt
A comparison of the efficacy of apathogenic genotype I (V4) and lentogenic genotype II (LaSota) strains of live Newcastle disease virus (NDV) vaccines was performed following vaccination with pathogen-associated molecular pattern (PAMP) H9N2 avian influenza vaccine and challenge with velogenic NDV genotype VII.1.1 (vNDV-VII.1.1). Eight groups (Gs) of day-old chicks were used (n = 25). Groups 1–4 received a single dose of PAMP-H9N2 subcutaneously, while Gs (1, 5) and (2, 6) received eye drops of V4 and LaSota, respectively, as two doses. All Gs, except for 4 and 8, were intramuscularly challenged with vNDV-VII.1.1 at 28 days of age. No signs were detected in Gs 1, 5, 4, and 8. The mortality rates were 0% in Gs 1, 4, 5, and 8; 40% in G2; 46.66% in G6; and 100% in Gs 3 and 7. Lesions were recorded as minimal in Gs 1 and 5, but mild to moderate in Gs 2 and 6. The lowest significant viral shedding was detected in Gs 1, 2, and 5. In conclusion, two successive vaccinations of broilers with a live V4 NDV vaccine provided higher protection against vNDV-VII.1.1 challenge than LaSota. PAMP-H9N2 with live NDV vaccines induced more protection than the live vaccine alone.
