Stem Cell Research (Oct 2017)

Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a modified RNA system

  • Kyung-Ok Uhm,
  • Gue Youn Go,
  • So-Jung Kim,
  • Eun Hee Jo,
  • Hye Young Choi,
  • Young Sam Im,
  • Hye-Yeong Ha,
  • Jung-Hyun Kim,
  • Soo Kyung Koo

DOI
https://doi.org/10.1016/j.scr.2017.02.009
Journal volume & issue
Vol. 24, no. C
pp. 148 – 150

Abstract

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We generated human induced pluripotent stem cells (KSCBi002-B and KSCBi002-B-1) from the dermal fibroblasts of a donor using a modified RNA-based gene delivery method. According to GTG-banding analysis, the generated KSCBi002-B line has a cytogenetic abnormality (46,XY, t(1;4)(q21;q25)) that is distinct from that of the donor, whereas KSCBi002-B-1 has a normal karyotype (46,XY). These cell lines can be useful as a model for characterizing the hiPSCs generated by a non-viral and non-integrative system, or as a chromosomal balanced translocation model. These two cell lines are registered and available from the National Stem Cell Bank, Korea National Institute of Health.