Decreased m6A Modification of CD34/CD276(B7-H3) Leads to Immune Escape in Colon Cancer

Yiran Zhou; Haodong Zhou; Jianlin Shi; Aoran Guan; Yankun Zhu; Zongliu Hou; Ruhong Li

doi:10.3389/fcell.2021.715674

Frontiers in Cell and Developmental Biology (Jul 2021)

Decreased m6A Modification of CD34/CD276(B7-H3) Leads to Immune Escape in Colon Cancer

Yiran Zhou,
Haodong Zhou,
Jianlin Shi,
Aoran Guan,
Yankun Zhu,
Zongliu Hou,
Ruhong Li

Affiliations

Yiran Zhou: Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, First Department of General Surgery, Yan’an Affiliated Hospital of Kunming Medical University, Kunming, China
Haodong Zhou: Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, First Department of General Surgery, Yan’an Affiliated Hospital of Kunming Medical University, Kunming, China
Jianlin Shi: Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, Department of Thoracic Surgery, Yan’an Affiliated Hospital of Kunming Medical University, Kunming, China
Aoran Guan: Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, First Department of General Surgery, Yan’an Affiliated Hospital of Kunming Medical University, Kunming, China
Yankun Zhu: Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, First Department of General Surgery, Yan’an Affiliated Hospital of Kunming Medical University, Kunming, China
Zongliu Hou: Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, Kunming, China
Ruhong Li: Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, First Department of General Surgery, Yan’an Affiliated Hospital of Kunming Medical University, Kunming, China

DOI: https://doi.org/10.3389/fcell.2021.715674
Journal volume & issue: Vol. 9

Abstract

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Previous studies have reported that m6a modification promotes tumor immune escape by affecting tumor microenvironment (TME). Due to the complexity of TME, a single biomarker is insufficient to describe the complex biological characteristics of tumor and its microenvironment. Therefore, it is more meaningful to explore a group of effective biomarkers reflecting different characteristics of cancer to evaluate the biological characteristics of solid tumors. Here, the immune gene CD34/CD276 with different m6A peak was obtained by m6A sequencing (MeRIP-seq) of colon cancer (CRC)clinical samples and combined with MsIgDB database, which was used to perform cluster analysis on TCGA-COAD level 3 data. The CD34/CD276 as a molecular marker for CRC prognosis was confirmed by survival analysis and immunohistochemical assay. Further bioinformatics analysis was carried out to analyze the molecular mechanism of CD34/CD276 affecting the TME through m6a-dependent down-regulation and ultimately promoting immune escape of CRC.

Published in Frontiers in Cell and Developmental Biology

ISSN: 2296-634X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.frontiersin.org/journals/cell-and-developmental-biology

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