Repair effect of human amniotic mesenchymal stem cells on uterine scars in rats

Abstract

Objective To exploring the effect of human amniotic mesenchymal stem cells(hAMSCs) on the repair of rat uterine scars. Methods The hAMSCs were isolated and cultured,Female SPF grade SD rats were selected for full-thickness incision of uterine wall and then implanted with hAMSCs. On the 30th day after operation, the uterine incision was examined histologically.ImageJ image analysis software was used to analyze and to compare the thickness of uterine myometrium and the percentage of fibrotic area in each group.Immunohistochemical method was used for detecting the percentage of positive areas of α-SMA, TGF-β1, and Ki-67. Results Compared with the PBS group, the hAMSCs group showed significant thickening of the uterine muscle layer and fibrotic area was decreased, The positive expression of α-SMA, and Ki-67 significantly increased(P＜0.05), while the expression of TGF-β1 was significantly reduced(P＜0.05). Conclusions The hAMSCs may promote the repair of uterine incision scars by reducing the formation of scar fibrosis and promoting the proliferation of smooth muscle cells in uterine scars.

Keywords

• human amniotic mesenchymal stem cell|cesarean section|uterine scar