Study on toxigenic fungi in ruminant feeds under desert conditions with special references to its biological control

Abstract

A total of 435 samples from feedstuff (130 samples of commercial ration feed storage from 1–30 days, 77 samples of commercial ration stored higher than 30 days, 57 samples from each of, derris, grind mixture, Tibn and wheat bran) were collected from the feed store houses of private farms located at the desert regions of Ras Sudr at South Sinai and Elameria area at Alexandria Governorate, from December 2012 to May 2014. The collected samples were analyzed for fungal growth. The results revealed that, the main moulds observed in the ruminant feeds were Penicillium spp., Aspergillus (A) flavus, Cladosporium spp., Mucor spp., Trichoderma spp., A. niger, Alternaria spp., Rhizopus spp., Fusarium spp., A. fumigates and A. terreus. In addition, the winter season was of higher incidence for moulds isolation than summer season. The most toxigenic aflatoxins secreted by Aspergillus flavus include Aflatoxin B1, Aflatoxin B2, Aflatoxin G1 and Aflatoxin G2. The results of biological treatment of Aflatoxins using Saccharomyces (S) cerevisiae, showed that, the addition of Saccharomyces cerevisiae at a level of (1 × 106) cfu.ml−1 and (1 × 109) cfu.ml−1 decreased the level of concentration of aflatoxin B1, B2, G1 and G2 and the level of (1 × 109) cfu.ml−1 was more efficient in reducing aflatoxins than the lower concentration.

Keywords

• Aflatoxin
• Saccharomyces
• Biocontrol
• Mycotoxins
• Feedstuff
• Egypt
• Aspergillus flavus