Comparison of classical and flowcytometric osmotic fragility and flowcytometric eosin-5-maleimide binding tests in diagnosis of hereditary spherocytosis

Abstract

Hereditary spherocytosis (HS) is the most common congenital hemolytic anemia in Northern Europe and North America. Damaged or deficient proteins in the erythrocyte membrane cause the condition, leading to a decrease in the surface area and volume of erythrocytes. Specialists traditionally use the osmotic fragility test (C-OF) for diagnosis. Researchers have developed new methods for flow cytometry-based tests that are the eosin-5-maleimide binding (EMA) and osmotic fragility (FC-OF) tests. In this study, we aimed to determine and compare the power of discrimination of FC-OF, EMA, and C-OF tests. Another purpose is to investigate the effect of incubation.

Keywords

• hereditary spherocytosis
• flow cytometry
• flow cytometric osmotic fragility test
• eosin-5-maleimide binding test
• eosin-5-maleimide
• osmotic fragility