Nature Communications (Aug 2021)
Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids
- Ann-Na Cho,
- Yoonhee Jin,
- Yeonjoo An,
- Jin Kim,
- Yi Sun Choi,
- Jung Seung Lee,
- Junghoon Kim,
- Won-Young Choi,
- Dong-Jun Koo,
- Weonjin Yu,
- Gyeong-Eon Chang,
- Dong-Yoon Kim,
- Sung-Hyun Jo,
- Jihun Kim,
- Sung-Yon Kim,
- Yun-Gon Kim,
- Ju Young Kim,
- Nakwon Choi,
- Eunji Cheong,
- Young-Joon Kim,
- Hyunsoo Shawn Je,
- Hoon-Chul Kang,
- Seung-Woo Cho
Affiliations
- Ann-Na Cho
- Department of Biotechnology, Yonsei University
- Yoonhee Jin
- Department of Biotechnology, Yonsei University
- Yeonjoo An
- Department of Biotechnology, Yonsei University
- Jin Kim
- Department of Biotechnology, Yonsei University
- Yi Sun Choi
- Department of Biotechnology, Yonsei University
- Jung Seung Lee
- Department of Biotechnology, Yonsei University
- Junghoon Kim
- Department of Biotechnology, Yonsei University
- Won-Young Choi
- Department of Biochemistry, Yonsei University
- Dong-Jun Koo
- Institute of Molecular Biology and Genetics, Seoul National University
- Weonjin Yu
- Signature Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School
- Gyeong-Eon Chang
- Department of Biotechnology, Yonsei University
- Dong-Yoon Kim
- Institute of Molecular Biology and Genetics, Seoul National University
- Sung-Hyun Jo
- Department of Chemical Engineering, Soongsil University
- Jihun Kim
- Division of Pediatric Neurology, Department of Pediatrics, Severance Children’s Hospital, Yonsei University College of Medicine
- Sung-Yon Kim
- Institute of Molecular Biology and Genetics, Seoul National University
- Yun-Gon Kim
- Department of Chemical Engineering, Soongsil University
- Ju Young Kim
- Department of Advanced Materials Engineering, Kangwon National University
- Nakwon Choi
- Brain Science Institute, Korea Institute of Science and Technology (KIST)
- Eunji Cheong
- Department of Biotechnology, Yonsei University
- Young-Joon Kim
- Department of Biochemistry, Yonsei University
- Hyunsoo Shawn Je
- Signature Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School
- Hoon-Chul Kang
- Division of Pediatric Neurology, Department of Pediatrics, Severance Children’s Hospital, Yonsei University College of Medicine
- Seung-Woo Cho
- Department of Biotechnology, Yonsei University
- DOI
- https://doi.org/10.1038/s41467-021-24775-5
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 23
Abstract
Brain organoids derived from human pluripotent stem cells can model human brain development and disease, though current culture systems fail to ensure reliable production of high-quality organoids. Here the authors combine human brain extracellular matrix and culture in a microfluidic device to promote structural and functional maturation of human brain organoids.
