Nature Communications (Nov 2018)

Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases

  • Jifu Duan,
  • Moritz Senger,
  • Julian Esselborn,
  • Vera Engelbrecht,
  • Florian Wittkamp,
  • Ulf-Peter Apfel,
  • Eckhard Hofmann,
  • Sven T. Stripp,
  • Thomas Happe,
  • Martin Winkler

DOI
https://doi.org/10.1038/s41467-018-07140-x
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 11

Abstract

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[FeFe]-hydrogenases catalyze H2-evolution and -oxidation at very high turnover-rates. Here the authors provide experimental evidence for the proposed proton-transfer (PT) pathway by kinetically, spectroscopically, and crystallographically characterizing eleven mutants from the two [FeFe]-hydrogenases CpI and HydA1.