Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat

Chengzhi Jiang; Yujie Luo; Doudou Huang; Meiling Chen; Ennian Yang; Guangrong Li; Zujun Yang

doi:10.3390/plants14101538

Plants (May 2025)

Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat

Chengzhi Jiang,
Yujie Luo,
Doudou Huang,
Meiling Chen,
Ennian Yang,
Guangrong Li,
Zujun Yang

Affiliations

Chengzhi Jiang: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
Yujie Luo: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
Doudou Huang: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
Meiling Chen: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
Ennian Yang: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
Guangrong Li: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
Zujun Yang: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China

DOI: https://doi.org/10.3390/plants14101538
Journal volume & issue: Vol. 14, no. 10
p. 1538

Abstract

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Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a highly destructive disease prevalent across most wheat-growing regions globally. The most effective strategy for combating this disease is through the exploitation of durable and robust resistance genes from the relatives of wheat. Thinopyrum intermedium (Host) Barkworth and D.R. Dewey has been widely hybridized with common wheat and has been shown to be a valuable source of genes, conferring resistance and tolerance against both the biotic and abiotic stresses affecting wheat. In this study, a novel wheat–Th. intermedium 2StS.2JSL addition line, named Th93-1-6, which originated from wheat–Th. intermedium partial amphidiploid line, Th24-19-5, was comprehensively characterized using nondenaturing-fluorescence in situ hybridization (ND-FISH) and Oligo-FISH painting techniques. To detect plants with the transfer of resistance genes from Th93-1-6 to wheat chromosomes, 2384 M1-M3 plants from the cross between Th93-1-6 and the susceptible wheat cultivar MY11 were studied by ND-FISH using multiple probes. A total of 37 types of 2StS.2JSL chromosomal aberrations were identified. Subsequently, 12 homozygous lines were developed to construct a cytological bin map. Ten chromosomal bins on the 2StS.2JSL chromosome were constructed based on 84 specific molecular markers. Among them, eight alien chromosome aberration lines, which all contained the bin 2StS-3, showed enhanced stripe rust resistance. Consequently, the gene(s) for stripe rust resistance was physically mapped to the 92.88-155.32 Mb region of 2StS in Thinopyrum intermedium reference genome sequences v2.1. Moreover, these newly developed wheat–Th. intermedium 2StS.2JSL translocation lines are expected to serve as valuable genetic resources in the breeding of rust-resistant wheat cultivars.

Published in Plants

ISSN: 2223-7747 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany
Website: http://www.mdpi.com/journal/plants

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