Mechanistic insights into the three steps of poly(ADP-ribosylation) reversal

Johannes Gregor Matthias Rack; Qiang Liu; Valentina Zorzini; Jim Voorneveld; Antonio Ariza; Kourosh Honarmand Ebrahimi; Julia M. Reber; Sarah C. Krassnig; Dragana Ahel; Gijsbert A. van der Marel; Aswin Mangerich; James S. O. McCullagh; Dmitri V. Filippov; Ivan Ahel

doi:10.1038/s41467-021-24723-3

Nature Communications (Jul 2021)

Mechanistic insights into the three steps of poly(ADP-ribosylation) reversal

Johannes Gregor Matthias Rack,
Qiang Liu,
Valentina Zorzini,
Jim Voorneveld,
Antonio Ariza,
Kourosh Honarmand Ebrahimi,
Julia M. Reber,
Sarah C. Krassnig,
Dragana Ahel,
Gijsbert A. van der Marel,
Aswin Mangerich,
James S. O. McCullagh,
Dmitri V. Filippov,
Ivan Ahel

Affiliations

Johannes Gregor Matthias Rack: Sir William Dunn School of Pathology, University of Oxford
Qiang Liu: Leiden University, Leiden Institute of Chemistry
Valentina Zorzini: Sir William Dunn School of Pathology, University of Oxford
Jim Voorneveld: Leiden University, Leiden Institute of Chemistry
Antonio Ariza: Sir William Dunn School of Pathology, University of Oxford
Kourosh Honarmand Ebrahimi: Department of Chemistry, University of Oxford, Chemistry Research Laboratory
Julia M. Reber: Molecular Toxicology Group, Department of Biology, University of Konstanz
Sarah C. Krassnig: Molecular Toxicology Group, Department of Biology, University of Konstanz
Dragana Ahel: Sir William Dunn School of Pathology, University of Oxford
Gijsbert A. van der Marel: Leiden University, Leiden Institute of Chemistry
Aswin Mangerich: Molecular Toxicology Group, Department of Biology, University of Konstanz
James S. O. McCullagh: Department of Chemistry, University of Oxford, Chemistry Research Laboratory
Dmitri V. Filippov: Leiden University, Leiden Institute of Chemistry
Ivan Ahel: Sir William Dunn School of Pathology, University of Oxford

DOI: https://doi.org/10.1038/s41467-021-24723-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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PARG and ARH3 are the main hydrolases to reverse serine poly(ADP-ribosylation) yet their activities in the process differ. Here, the authors synthesise linear and branched poly(ADP-ribose) molecules, perform structure-function analysis and elucidate the mechanistic differences between PARG and ARH3.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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