Impact of improper sample handling on Cobas CT/NG testing platform with dual swab sample collection kit for detecting Chlamydia trachomatis and Neisseria gonorrhoeae

Abstract

ABSTRACT In the realm of molecular diagnostics, accurate and reliable test results are imperative for effective clinical decision-making. This study delves into the effects of non-compliant sample collection practices on the Cobas CT/NG testing platform. Deviating from the manufacturer’s recommendation of single swab collection, our laboratory received dual swabs during sample collection. Subsequently, we embarked on an investigation to ascertain the cause behind a noticeable rise in invalid results, which were found to be exacerbated by improper sample handling practices, accounting for a significant 30% of cases. Through a comprehensive comparative analysis, we found that the use of dual swabs in testing contributed to a substantial increase in both invalid and false-negative results when compared to properly collected samples submitted with a single swab. This study underscores the importance of adhering to recommended protocols for sample collection to ensure accurate and reliable molecular diagnostic outcomes. IMPORTANCE The importance of this observation lies in its potential to directly impact testing outcomes and patient care. By identifying improper sample handling as a contributing factor to a substantial number of invalid results, we emphasize the need for meticulous adherence to recommended protocols during sample collection. Laboratories that overlook or are unaware of such deviations may inadvertently compromise the reliability and efficacy of their diagnostic processes, leading to misdiagnoses, delayed treatment, and patient dissatisfaction.

Keywords

• Cobas
• CT/NG
• duel swab
• invalid