NeuroImage (Sep 2020)
Amygdalar nuclei and hippocampal subfields on MRI: Test-retest reliability of automated volumetry across different MRI sites and vendors
- Giulia Quattrini,
- Michela Pievani,
- Jorge Jovicich,
- Marco Aiello,
- Núria Bargalló,
- Frederik Barkhof,
- David Bartres-Faz,
- Alberto Beltramello,
- Francesca B. Pizzini,
- Olivier Blin,
- Regis Bordet,
- Massimo Caulo,
- Manos Constantinides,
- Mira Didic,
- Antonios Drevelegas,
- Antonio Ferretti,
- Ute Fiedler,
- Piero Floridi,
- Hélène Gros-Dagnac,
- Tilman Hensch,
- Karl-Titus Hoffmann,
- Joost P. Kuijer,
- Renaud Lopes,
- Camillo Marra,
- Bernhard W. Müller,
- Flavio Nobili,
- Lucilla Parnetti,
- Pierre Payoux,
- Agnese Picco,
- Jean-Philippe Ranjeva,
- Luca Roccatagliata,
- Paolo M. Rossini,
- Marco Salvatore,
- Peter Schonknecht,
- Björn H. Schott,
- Julien Sein,
- Andrea Soricelli,
- Roberto Tarducci,
- Magda Tsolaki,
- Pieter J. Visser,
- Jens Wiltfang,
- Jill C. Richardson,
- Giovanni B. Frisoni,
- Moira Marizzoni
Affiliations
- Giulia Quattrini
- Laboratory of Alzheimer’s Neuroimaging and Epidemiology (LANE), IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy; Corresponding author. IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Via Pilastroni 4, 25125, Brescia, Italy.
- Michela Pievani
- Laboratory of Alzheimer’s Neuroimaging and Epidemiology (LANE), IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy
- Jorge Jovicich
- Center for Mind Brain Sciences, University of Trento, Trento, Italy
- Marco Aiello
- IRCCS SDN, Napoli, Italy
- Núria Bargalló
- Department of Neuroradiology and Image Research Platform, Hospital Clínic de Barcelona, IDIBAPS, Barcelona, Spain
- Frederik Barkhof
- Department of Radiology and Nuclear Medicine, VU University Medical Center, Amsterdam, the Netherlands; Queen Square Institute of Neurology and Centre for Medical Image Computing, University College London, UK
- David Bartres-Faz
- Department of Medicine and Health Sciences, Faculty of Medicine, Universitat de Barcelona and IDIBAPS, Barcelona, Spain
- Alberto Beltramello
- Department of Radiology, IRCCS “Sacro Cuore-Don Calabria”, Negrar, Verona, Italy
- Francesca B. Pizzini
- Radiology, Department of Diagnostic and Public Health, University of Verona, Verona, Italy
- Olivier Blin
- Aix-Marseille University, UMR-INSERM 1106, Service de Pharmacologie Clinique, APHM, Marseille, France
- Regis Bordet
- Aix-Marseille Université, INSERM U 1106, 13005, Marseille, France
- Massimo Caulo
- University “G. d’Annunzio” of Chieti, Chieti, Italy
- Manos Constantinides
- Interbalkan Medical Centre of Thessaloniki, Thessaloniki, Greece
- Mira Didic
- Aix-Marseille Université, Inserm, Institut de Neurosciences des Systèmes (INS) UMR_S 1106, 13005, Marseille, France; APHM, Timone, Service de Neurologie et Neuropsychologie, Hôpital Timone Adultes, Marseille, France
- Antonios Drevelegas
- Interbalkan Medical Centre of Thessaloniki, Thessaloniki, Greece
- Antonio Ferretti
- University “G. d’Annunzio” of Chieti, Chieti, Italy
- Ute Fiedler
- Institutes and Clinics of the University Duisburg-Essen, Essen, Germany
- Piero Floridi
- Perugia General Hospital, Neuroradiology Unit, Perugia, Italy
- Hélène Gros-Dagnac
- ToNIC, Toulouse NeuroImaging Center, Université de Toulouse, Inserm, UPS, France
- Tilman Hensch
- Department of Psychiatry and Psychotherapy, University of Leipzig Medical Center, Leipzig, Germany
- Karl-Titus Hoffmann
- Department of Neuroradiology, University Hospital Leipzig, Leipzig, Germany
- Joost P. Kuijer
- Department of Radiology and Nuclear Medicine, VU University Medical Center, Amsterdam, the Netherlands
- Renaud Lopes
- INSERM U1171, Neuroradiology Department, University Hospital, Lille, France
- Camillo Marra
- Catholic University, Fondazione Policlinico A. Gemelli, IRCCS, Rome, Italy
- Bernhard W. Müller
- LVR-Hospital Essen, Department for Psychiatry and Psychotherapy, Faculty of Medicine, University of Duisburg-Essen, Germany
- Flavio Nobili
- Department of Neuroscience (DINOGMI), University of Genoa, Genoa, Italy; IRCCS, Ospedale Policlinico San Martino, Genova, Italy
- Lucilla Parnetti
- Section of Neurology, Department of Medicine, University of Perugia, Perugia, Italy
- Pierre Payoux
- ToNIC, Toulouse NeuroImaging Center, Université de Toulouse, Inserm, UPS, France
- Agnese Picco
- Department of Neuroscience (DINOGMI), University of Genoa, Genoa, Italy
- Jean-Philippe Ranjeva
- CRMBM-CEMEREM, UMR 7339, Aix-Marseille University, CNRS, Marseille, France
- Luca Roccatagliata
- IRCCS, Ospedale Policlinico San Martino, Genova, Italy; Department of Health Science (DISSAL), University of Genoa, Genoa, Italy
- Paolo M. Rossini
- Dept. Neuroscience & Rehabilitation, IRCCS San Raffaele-Pisana, Rome, Italy
- Marco Salvatore
- IRCCS SDN, Napoli, Italy
- Peter Schonknecht
- Department of Psychiatry and Psychotherapy, University of Leipzig Medical Center, Leipzig, Germany
- Björn H. Schott
- Department of Psychiatry and Psychotherapy, University Medical Center Goettingen (UMG), Göttingen, Germany; Leibniz Institute for Neurobiology, Magdeburg, Germany; German Center for Neurodegenerative Diseases (DZNE), Goettingen, Germany
- Julien Sein
- CRMBM-CEMEREM, UMR 7339, Aix-Marseille University, CNRS, Marseille, France
- Andrea Soricelli
- IRCCS SDN, Napoli, Italy
- Roberto Tarducci
- Perugia General Hospital, Medical Physics Unit, Perugia, Italy
- Magda Tsolaki
- Aristotle University of Thessaloniki, Thessaloniki, Greece
- Pieter J. Visser
- Department of Neurology, Alzheimer Centre, VU Medical Centre, Amsterdam, Netherlands; Maastricht University, Maastricht, Netherlands
- Jens Wiltfang
- Department of Psychiatry and Psychotherapy, University Medical Center Goettingen (UMG), Göttingen, Germany; Neurosciences and Signaling Group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, Aveiro, Portugal; German Center for Neurodegenerative Diseases (DZNE), Goettingen, Germany
- Jill C. Richardson
- Neurosciences Therapeutic Area, GlaxoSmithKline R&D, Gunnels Wood Road, Stevenage, United Kingdom
- Giovanni B. Frisoni
- Laboratory of Alzheimer’s Neuroimaging and Epidemiology (LANE), IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy; Memory Clinic and LANVIE-Laboratory of Neuroimaging of Aging, Hospitals and University of Geneva, Geneva, Switzerland
- Moira Marizzoni
- Laboratory of Alzheimer’s Neuroimaging and Epidemiology (LANE), IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy
- Journal volume & issue
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Vol. 218
p. 116932
Abstract
Background: The amygdala and the hippocampus are two limbic structures that play a critical role in cognition and behavior, however their manual segmentation and that of their smaller nuclei/subfields in multicenter datasets is time consuming and difficult due to the low contrast of standard MRI. Here, we assessed the reliability of the automated segmentation of amygdalar nuclei and hippocampal subfields across sites and vendors using FreeSurfer in two independent cohorts of older and younger healthy adults. Methods: Sixty-five healthy older (cohort 1) and 68 younger subjects (cohort 2), from the PharmaCog and CoRR consortia, underwent repeated 3D-T1 MRI (interval 1–90 days). Segmentation was performed using FreeSurfer v6.0. Reliability was assessed using volume reproducibility error (ε) and spatial overlapping coefficient (DICE) between test and retest session. Results: Significant MRI site and vendor effects (p 0.43, p 0.80). Conclusion: Our results support the use of volumetric measures of larger amygdalar nuclei and hippocampal subfields in multisite MRI studies. These measures could be useful for disease tracking and assessment of efficacy in drug trials.
