Fourier Transform Infrared (FTIR) Spectroscopic Study of Biofilms Formed by the Rhizobacterium <i>Azospirillum baldaniorum</i> Sp245: Aspects of Methodology and Matrix Composition
Alexander A. Kamnev,
Yulia A. Dyatlova,
Odissey A. Kenzhegulov,
Yulia P. Fedonenko,
Stella S. Evstigneeva,
Anna V. Tugarova
Affiliations
Alexander A. Kamnev
Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms—Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, Russia
Yulia A. Dyatlova
Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms—Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, Russia
Odissey A. Kenzhegulov
Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms—Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, Russia
Yulia P. Fedonenko
Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms—Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, Russia
Stella S. Evstigneeva
Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms—Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, Russia
Anna V. Tugarova
Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms—Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, Russia
Biofilms represent the main mode of existence of bacteria and play very significant roles in many industrial, medical and agricultural fields. Analysis of biofilms is a challenging task owing to their sophisticated composition, heterogeneity and variability. In this study, biofilms formed by the rhizobacterium Azospirillum baldaniorum (strain Sp245), isolated biofilm matrix and its macrocomponents have for the first time been studied in detail, using Fourier transform infrared (FTIR) spectroscopy, with a special emphasis on the methodology. The accompanying novel data of comparative chemical analyses of the biofilm matrix, its fractions and lipopolysaccharide isolated from the outer membrane of the cells of this strain, as well as their electrophoretic analyses (SDS-PAGE) have been found to be in good agreement with the FTIR spectroscopic results.
