Nature Communications (Mar 2021)

A designer FG-Nup that reconstitutes the selective transport barrier of the nuclear pore complex

  • Alessio Fragasso,
  • Hendrik W. de Vries,
  • John Andersson,
  • Eli O. van der Sluis,
  • Erik van der Giessen,
  • Andreas Dahlin,
  • Patrick R. Onck,
  • Cees Dekker

DOI
https://doi.org/10.1038/s41467-021-22293-y
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

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Intrinsically disordered FG-Nups line the Nuclear Pore Complex (NPC) lumen and form a selective barrier where transport of most proteins is inhibited, whereas specific transporter proteins are able to pass. Here, the authors reconstitute the selective behaviour of the NPC by introducing a rationally designed artificial FG-Nup that demonstrates that no specific spacer sequence nor a spatial segregation of different FG-motif types are needed to create selective NPCs.