miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation

Yani Zhang; Wenhui Zhang; Cai Hu; Yingjie Wang; Man Wang; Qisheng Zuo; Ahmed Kamel Elsayed; Yi Li; Bichun Li

doi:10.1155/2021/5546936

Stem Cells International (Jan 2021)

miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation

Yani Zhang,
Wenhui Zhang,
Cai Hu,
Yingjie Wang,
Man Wang,
Qisheng Zuo,
Ahmed Kamel Elsayed,
Yi Li,
Bichun Li

Affiliations

Yani Zhang: College of Animal Science and Technology, Yangzhou University, Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, Yangzhou, 225009 Jiangsu, China
Wenhui Zhang: College of Animal Science and Technology, Yangzhou University, Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, Yangzhou, 225009 Jiangsu, China
Cai Hu: College of Animal Science and Technology, Yangzhou University, Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, Yangzhou, 225009 Jiangsu, China
Yingjie Wang: College of Animal Science and Technology, Yangzhou University, Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, Yangzhou, 225009 Jiangsu, China
Man Wang: College of Animal Science and Technology, Yangzhou University, Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, Yangzhou, 225009 Jiangsu, China
Qisheng Zuo: College of Animal Science and Technology, Yangzhou University, Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, Yangzhou, 225009 Jiangsu, China
Ahmed Kamel Elsayed: Faculty of Veterinary Medicine, Suez Canal University, 41522 Ismailia, Egypt
Yi Li: College of Computer Science and Technology, Wenzhou-Kean University, Wenzhou, 325035 Zhejiang, China
Bichun Li: College of Animal Science and Technology, Yangzhou University, Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, Yangzhou, 225009 Jiangsu, China

DOI: https://doi.org/10.1155/2021/5546936
Journal volume & issue: Vol. 2021

Abstract

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MicroRNAs (miRNAs) are essential factors in the reproductive process of poultry. Here, we found miR-302d is a potential differentiation and negative factor of chicken embryonic stem cells (ESCs) into spermatogonia stem cells (SSCs). The competition mechanism was carried out for the preliminary exploration to determine the relationship among miR-302d, lncRNA-341(interacting with miR-302d), and target gene TLE4. The results showed that lncRNA-341 can competitively bind to miR-302d to decrease the targeted binding of miR-302d and TLE4 which promotes the differentiation of chicken SSCs. Moreover, it is suggested that miR-302d may participate in the Wnt signaling pathway through TLE4.

Published in Stem Cells International

ISSN: 1687-966X (Print); 1687-9678 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine
Website: https://onlinelibrary.wiley.com/journal/4162

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