An insight on molecular diversity in ricebean (Vigna umbellata) genotypes using microsatellite markers

Abstract

A total of 25 adzukibean derived SSR markers were used for molecular diversity analysis among 45 ricebean genotypes and a greengram cultivar, CO 7. Cross species amplification was observed for all the markers in ricebean. Number of alleles, in the present study, varied from one to three. In ricebean, the allele size ranged from 110 to 250 bp, whereas in greengram cultivar, it ranged from 110 to 250 bp. PIC values ranged from 0.198 to 0.689. Out of 25 markers, 13 markers were found to be polymorphic among ricebean genotypes. Apart from this, 13 markers differentiated the ricebean genotypes from the greengram cultivar, CO 7. Dissimilarity values between the ricebean genotypes and the greengram cultivar, CO 7 were greater when compared to the dissimilarity values within the ricebean genotypes. Dendrogram and the tree generated based on Ward’s and neighbor joining methods classified the genotypes into five clusters and three groups, respectively.

Keywords

• ssr
• rice bean
• diversity
• cross species amplification