Vascular Health and Risk Management (Dec 2020)

Expression of Hypoxia-Inducible Factor-1α (HIF1A) and Lp-PLA2 in Low, Intermediate, and High Cardiovascular Disease Risk Population

  • Heriansyah T,
  • Chomsy IN,
  • Kumboyono K,
  • Pratiwi PA,
  • Wihastuti TA

Journal volume & issue
Vol. Volume 16
pp. 507 – 513

Abstract

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Teuku Heriansyah,1 Indah Nur Chomsy,2 Kumboyono Kumboyono,3 Pinandita Annisa Pratiwi,4 Titin Andri Wihastuti5 1Department of Cardiology and Vascular Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia; 2Master Program in Biomedical Science, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia; 3Nursing Department, Faculty of Medicine, University of Brawijaya, Malang 65145, Indonesia; 4Medical Study Program, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia; 5Department of Biomedicine, Faculty of Medicine, Brawijaya University, Malang 65145, IndonesiaCorrespondence: Teuku HeriansyahDepartment of Cardiology and Vascular Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaEmail [email protected]: The pathomechanism of CVD is a complex and multifactorial process. The primary mechanism of CVD is atherosclerosis. Inflammation in atherogenesis raises the risk of hypoxia, which will activate hypoxia-inducible factor-1α (HIF1A). Also, together with lipoprotein-associated phospholipase A2 (Lp-PLA2), an inflammatory mediator for atherogenesis.Purpose: This study aims to measure the hypoxia-inducible factor-1α (HIF1A) expression and its correlation to Lp-PLA2 expression at low-risk, intermediate, and high-risk CVD populations.Patients and Methods: The study used a correlational analysis method with a total sampling technique in 160 individuals in the risk population. The atherosclerosis risk group was analyzed using the Framingham Risk Score and categorized into low, intermediate, and high-risk groups. Venous blood samples taken from respondents were measured using the ELISA method with Lp-PLA2 and HIF-1α as parameters. Data were analyzed using normality test, homogeneity test, one-way ANOVA, post hoc–Tukey HSD, and Pearson correlation.Results: The concentration of HIF1A had a very strong correlation with Lp-PLA2 expression, both in the low-risk group (r = 0.512), intermediate (r = 0.512), and high (r = 0.715) (P < 0.05). However, the concentrations of Lp-PLA2 did not match the FRS.Conclusion: HIF1A expression increased with increasing risk, while Lp-PLA2 expression decreased with increasing risk of atherosclerosis based on the FRS category. There is a significant correlation between HIF1A expression and Lp-PLA2 expression based on FRS.Keywords: cardiovascular disease, atherosclerosis, HIF1A, Lp-PLA2, Framingham Risk Score

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