JATROPHA MOLLISSIMA EXTRACT WITH CHITOSAN/COLLAGEN SCAFFOLDS AND THEIR BIODEGRADABILITY EVALUATION

Abstract

Scaffolds are devices with interconnected pores that favor cell interaction and proliferation. They play a significant role in tissue engineering, as they mimic the conditions of tissue production in vivo. The aim of this study was to synthesize scaffolds of chitosan, collagen, and Jatropha mollissima by lyophilization and characterize their chemical/biodegradability and morphological properties. For that, techniques of optical microscopy, infrared spectrometry, biodegradation test, and degree of swelling were performed. A 2% (m/v) chitosan/collagen (1:9) in lactic acid solution was produced. Then, Jatropha mollissima crude extract was added to this solution in different proportions of mass (5, 10, and 15%). Optical microscope images indicated the three-dimensional network formation with interconnected pores and predominantly lamellar shape. Through infrared spectrometry, characteristic bands indicated a physical mixture. Also, the swelling degree of all samples was proportional to the amount of Jatropha mollissima extract with the highest percentage of 1852%. Biodegradation test also revealed a proportional ratio between the amount of sap used and the degraded percentage with the lowest value of around 35% in 28 days. In conclusion, it can be inferred that the scaffolds produced show potential to be used in tissue engineering as dressings.

Keywords

• scaffolds
• chitosan
• collagen
• Jatropha mollissima.